Part:BBa_K3962340:Design

pBAD promoter with mCherry for inducible expression of reporter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1144
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961
  

Design Notes

We designed this construct to calibrate the expression of pBAD to a standardized constitutive promoter. Codon optimization was performed for the expression of the gene in E. coli.

Source

All subparts are from iGEM registry.